Figure 8

Model of GABP alpha/beta interaction with the UP site and its effect on BRCA1 promoter activity. Schematic representations of the regulation of the BRCA1 promoter. In both MCF-7 and T-47D cells (upper diagram) the RIBS and UP sites, respectively, are shown to bind GABP alpha/beta (shaded circles represent the DNA binding alpha subunit, with the beta subunit indicated by the dark line) while the E2F site has previously been suggested to bind E2F1, E2F4 and E2F6. The UP site also binds another unknown protein (oval) and together the UP and E2F sites are able to recruit an unknown co-repressor. The GA mutant of the UP site (x) disrupts binding of GABP alpha/beta while the US mutant affects the binding of the other unidentified factor. In T-47D cells this other factor does not appear to be functional (dashed oval). See text for explanation.