Figure 7

Cotransfection of the GABP alpha/beta expression vectors and the GABP alpha shRNA with RIBS and UP site multimer reporter constructs. MCF-7 and T-47D cells were transiently transfected with 125 ng of GF-TATA-renilla-luciferase reporter containing oligomerised RIBS or UP sites (RIBSn-pRL, UPn-pRL), 25 ng of internal control vector (CMV-Luc), and either 25 ng each of GABP alpha and beta expression constructs (light grey bars), or 50 ng of their empty control vector (pCAGGs; white bars), or 50 ng of GABP alpha shRNA (black bars) or its empty control vector (H1–2; dark grey bars). Cells were harvested 48 hours post-transfection and analysed for luciferase activity. The data presented are the mean values ± standard deviation of a representative experiment performed in triplicate and normalized as described in Figure 2. Statistical significance was determined using a t-test and significant results (p = 0.05) are indicated by asterisks and are in relation to the empty vector control for each condition (pCAGGS or H1–2).