In vitro MT-1 cell viability. The morphology of human MT-1 cells in vitro, expression of G3 in cell lysate and culture media, and tumor cell viability in serum-free and 1% FBS culture conditions for the G3 experimental group are compared to control. (a) Morphologically, cells in the MT-1 G3-transfected MT-1 cells appeared more elongated in vitro when compared to the predominant cuboid appearance of cells in the vector control group. (b) The expression of G3 in the cell lysate and culture media of MT-1 cells is demonstrated and is contrasted to the vector control group. (c) A greater tumor cell viability (mean cell number + SD) was observed in serum-free and (d) 1% FBS culture conditions for the G3 experimental group as compared with the vector control group (p < 0.05 days 5,6, and 7).