Effect of fibroblast conditioned on apoptosis and cell viability. For PMC42-LA cells cultured in fibroblast-conditioned medium, trypan blue dye exclusion was used to analyze cell viability, and total number of cells were counted and averaged to determine the average percentage of viable cells. (a) There was a slight variation in cell viability at 24 hours (75–100% viable cells [average 91.67 ± 14.43% in NMF-conditioned medium] and 85–100% [91.90 ± 7.33%] in CAF-conditioned medium). Similar cell viability was observed at 3 days (97–100% [99.21 ± 1.37%] in NMF-conditioned medium and 98–100% [average 99.40 ± 1.05%] in CAF-conditioned medium). There was no difference at 5 days (97–98% [98.48 ± 0.69%] in NMF-conditioned medium and 97–100% [98.93 ± 1.13%] in CAF-conditioned medium). (b) There was no significant difference in active caspase-3 expression between cultures. CAF, cancer-associated fibroblast; NMF, normal mammary fibroblast.