Cell supernatant analysis of angiogenic proteins revealed decreased levels with celecoxib treatment. The cell culture supernatants from MDA-MB-231 cells treated with vehicle and 40 mmol/l celecoxib for 24 hours were tested on a human angiogenesis protein array. The cell culture supernatants of cells treated with vehicle had higher amounts of GRO, IL-6, IL-8, TIMP1, TIMP2, and VEGF based on gray levels or brightness values as compared with cells treated with celecoxib. Other proteins affected by treatment were EGF, bFGF, and angiogenin. The array template is shown in the lower panel, with dark gray indicating high expression. bFGF, basic fibroblast growth factor; EGF, epidermal growth factor; IFN, interferon; IGF, insulin-like growth factor; IL, interleukin; MCP, monocyte chemoattractant protein; PDGF, platelet-derived growth factor; PG, prostaglandin; RANTES, regulated on activation, normal T cell expressed and secreted; TIMP, tissue inhibitor of matrix metalloproteinase; VEGF, vascular endothelial growth factor.