Proliferation is altered by MUC1 small interfering RNA (siRNA) in both cell lines. (a) [3H]Thymidine incorporation is shown by the level of radioactivity (cpm) emitted from cells after 24 hours incubation with radionucleotide. Results reflect the average of three independent experiments. *p < 0.006 compared to luciferase siRNA (siLuc) treated cells of the same line (that is, 468.siLuc or BT.siLuc). (b) Flow cytometric analysis of bromodeoxyuridine (BrdU) incorporation. siRNA-transfected cells were incubated with BrdU for 1.5 hours, and stained with a Phycoerythrin PE-conjugated antibody against BrdU. A representative experiment is shown. (c) Invasion of MDA-MB-468 cells in transwells plated with a panel of extracellular matrix proteins or no matrix (control). Representative results are shown as percent invasion, calculated as: number of invaded cells/total number of cells × 100.