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Table 2 Comparison of H4.77.16 and HFR1 staining patterns in the membrane, cytoplasm and nucleus

From: HER4 in breast cancer: comparison of antibodies against intra- and extra-cellular domains of HER4

   H4.77.16 Kappa value
   Negative Positive Total  
Membrane      0.426 (p < 0.001)
HFR1 Negative 273 28 301  
  Positive 10 18 28  
  Total 283 46 329  
Cytoplasmic      0.351 (p < 0.001)
HFR1 Negative 41 15 56  
  Positive 68 205 273  
  Total 109 220 329  
Nuclear      0.051 (p < 0.018)
HFR1 Negative 21 3 24  
  Positive 194 111 305  
  Total 215 114 329  
  1. Chi-square where numbers of cases negative or positive for each antibody at the membrane, cytoplasm or nucleus are shown using cut offs defined in the text. Cohen's Kappa value measures the agreement between the two antibodies, where a value of 1 indicates perfect agreement and a value of 0 indicates agreement is no better than chance.