Dose-response analyses of ibandronate on MCF-7, IBEP-2 and MDA-MB-231 cells. Breast cancer cells were cultured for three days in steroid-free medium containing ibandronate (Iban) or vehicle (control). Cell proliferation was determined by crystal violet staining assay. Data are presented as percentages of control values (mean ± SD). Experiments were performed four times in replicate. Asterisks indicate ANOVA p < 0.01 versus control, Dunnett post hoc test.