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Table 1 Antibodies used for immunohistochemical staining

From: Prognostic relevance of disseminated tumor cells in the bone marrow and biological factors of 265 primary breast carcinomas

Factor

Antibody

Companya

Dilution

Scoring

Cutoff

HER2

NCL-CB 11

Novocastra

1:40

0, 1, 2, 3

≥ 2+

Top IIa

Ab1, clone SWT3D1

Oncogene

1:40

% stained cells

>10%

Ki 67

Ki 67, clone MIB-1

Dako

1:100

% stained cells

>0%

p53

p53, clone DO-7

Dako

1:200

% stained cells

>5%

  1. HER2, human epithelial growth factor receptor 2; Top IIa, topoisomerase IIα.
  2. aNovocastra, Newcastle upon Tyne, UK; Oncogene, Cambridge, MA, USA; DAKO, Hamburg, Germany