Figure 4

Evaluation of candidate promoters for transcriptional targeting of primary breast cancer tissue slices. Human breast cancer tissue slices were obtained from primary breast cancer samples using the Krumdieck Tissue Slicer. Slices were infected with AdCXCR4Luc, AdSurvivinLuc, AdSLPILuc, AdEGP-2Luc, AdCox2MLucor AdCMVLuc at a multiplicity of infection of 500. Gene expression was measured 24 h after infection and is presented for the candidate promoters as percentage of cytomegalovirus promoter activity in relative light units (RLU). All data points are of triplicate slices; bars represent means ± standard deviation. Cox, cyclooxygenase; CXCR4, α-chemokine SDF-1 receptor; EGP, epithelial glycoprotein; SLPI, secretory leukoprotease inhibitor.