Figure 1

FISH analysis of breast cancer cells from patient B8. (a) Analysis with a specific LRIG1 (red) probe showing increased gene copy number (more than two copies) of the LRIG1 gene at 3p14. (b) Analysis with a specific CEP3 (centromeric chromosome 3; red) probe, showing no additional chromosome 3. (c) Analysis with a specific 3p subtelomeric probe (green) and LRIG1 (red) mixture showing increased gene copy number of the LRIG1 gene but only two copies of the 3p arm. (d) Analysis with a mixture of probes for CEP3 (red), X chromosome (green) and chromosome 18 (blue), showing no aneuploidy for these chromosomes.