Identification of key signaling components of SHBG-mediated pathway(s). A flow-chart of the experimental design and method (involving comparative proteomic analyses) for identification of key regulated signaling components in SHBG-mediated pathways in breast cancer cells. Each cell type will be exposed to SHBG (vs not exposed to SHBG), and to human estradiol (E2) (vs not exposed to E2). Differential analysis of proteomes for the different cell lines/treatments will then be carried out. Differences in expression/post-translational modifications of specific proteins will determine what proteins are of interest. End effects will be examined as well as the results of time-dependent experiments.