Effect of propofol-docosahexaenoic acid (propofol-DHA) and propofol-eicosapentaenoic (propofol-EPA) conjugates on cytochrome c release. MDA-MB-231 cells (0.5 × 106/well) were grown in 96-well plates for 24 hours at 37°C in a humidified CO2 incubator with control or test compounds. (a) Postnuclear supernatant was used for the determination of cytochrome c release using immunobloting as described in the Materials and Methods section. The relative distributions of cytochrome c and glyceraldehyde-3-phosphate dehydrogenase (loading control) were determined. (b) Quantification was by densitometry analysis with the Kodak imaging system. Results are means ± SEM for three experiments. The results were analyzed by analysis of variance and Dunnett's multiple comparison test to control the Type I experimental wise error. Significant differences from the control (P < 0.05) are indicated with an asterisk. D, 25 μM DHA; E, 25 μM EPA; P, 25 μM propofol; D+P, 25 μM DHA plus 25 μM propofol; E+P, 25 μM EPA plus 25 μM propofol; D-P, 25 μM propofol-DHA; E-P, 25 μM propofol-EPA.