Figure 7
Effect of propofol-docosahexaenoic acid (propofol-DHA) and propofol-eicosapentaenoic acid (propofol-EPA) conjugates on breast cancer cell apoptosis. MDA-MB-231 cells (104) were incubated with 25 μM DHA (D), 25 μM EPA (E), 25 μM propofol (P), 25 μM each of D+P, 25 μM each of E+P, 25 μM propofol-DHA (D-P) or 25 μM propofol-EPA (E-P) for 24 hours. The control cells (C) were treated with equal amounts of ethanol. The apoptotic cells were stained by using the Vybrant Apoptosis assay kit as described in the text. Results are means ± SEM for four experiments. The results were analyzed by analysis of variance and Dunnett's multiple comparison test to control the Type I experimental wise error. Significant differences from the control (P < 0.05) are indicated with an asterisk.