- Paper Report
- Open Access
Carbonic anhydrase expression in DCIS
- Rodolfo Laucirica1
© Biomed Central Ltd 2001
- Received: 9 May 2001
- Accepted: 20 August 2001
- Published: 1 December 2001
- Carbonic anhydrase, CA IX, CA XII, DCIS, hypoxia, MIB1, necrosis
Hypoxia has been associated with biologically aggressive tumors, possibly through upregulation of hypoxia-inducible genes. The authors recently identified two hypoxia identifiable genes, carbonic anhydrase (CA) CA IX and CA XII, in epithelial tumor cell lines. In this study, the researchers attempted to correlate expression of these genes with benign, preinvasive, and invasive carcinomas of the breast as well as with proliferative activity and mammographically detected calcification.
CA XII protein was found in normal breast tissue and benign lesions. CA IX protein expression was focally present and specifically associated with areas of necrosis and high grade lesions. In contrast, CA XII protein expression was typically homogeneous and associated with lack of necrosis and low grade lesions. Neither marker was significantly associated with proliferative activity. The authors demonstrated a significant relationship between mammographically detected calcification and reduced CA XII protein levels. Although no such relationship existed when correlating CA IX protein levels with calcification, there was a trend toward increased CA IX protein in those cases where linear-type calcifications were present. Finally, the presence of both markers in 29-71% of invasive lesions associated with ductal carcinoma in situ (DCIS) suggests that these CAs may also play a role in the development of invasive carcinomas of the breast.
Western blotting, immunohistochemistry
- Wykoff CC, Beasley N, Watson PH, Campo L, Chia SK, English R, Pastorek J, Sly WS, Ratcliffe P, Harris AL: Expression of the hypoxia-inducible and tumor-associated carbonic anhydrases in ductal carcinoma in situof the breast. Am J Pathol . 2001, 158: 1011-1019. View ArticlePubMedPubMed CentralGoogle Scholar