- Paper Report
- Open Access
Comparison of different methods of HER-2 analysis in archival tissue
- Richard de Boer1
© Biomed Central Ltd 2001
- Received: 9 April 2001
- Accepted: 20 August 2001
- Published: 1 December 2001
- FISH, HER-2 gene amplification, HER-2 overexpression, immunohistochemistry
Overexpression of the HER-2 oncogene is likely to be both a prognostic and a predictive factor in patients with breast cancer. There is considerable controversy over the best way to test for tumour HER-2 status. This study analysed HER-2 status in archival breast cancer tissue, by assessing the immunohistochemical (IHC) sensitivity of a series of antibodies. HER-2 status was also determined by fluorescence in situhybridisation (FISH) of amplified genes.
The HER-2 gene was amplified in 28% of the tumours, whereas overexpression was seen in 26-42% of the tumours. Strongly positive IHC results (3+) with any of the antibodies were always associated with gene amplification whilst weakly positive results (2+) often were not. The authors concluded that tumours showing high levels (3+) of overexpression on IHC testing almost certainly have gene amplification, but that FISH testing should be considered for tumours with lower levels of positive expression.
Immunohistochemistry, FISH, paraffin sections
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Jimenez RE, Wallis T, Tabasczka P, Visscher DW: Determination of Her-2/Neu status in breast carcinoma: comparative analysis of immunohistochemistry and fluorescent in situ hybridisation [see comments]. Mod Pathol 2000, 13:37-45 (PubMed abstract).