- Paper Report
- Open Access
Benzo[a]pyrene downregulation of BRCA1
- Chris Jones
© Current Science Ltd 1999
- Published: 1 December 1999
- Aromatic hydrocarbon receptor
- sporadic breast cancer
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants found in tobacco smoke, industrial pollution, coal tar and car exhaust. The PAH benzo[a]pyrene (B[a]P), a classic DNA-damaging carcinogen found in high concentrations in tobacco smoke, displays a high affinity for the aromatic hydrocarbon receptor (AhR). Population studies have documented that smokers have more PAH-DNA adducts than do non-smokers, and suggest a potential correlation between tobacco smoking and the development of breast cancer.
To investigate the effects of PAHs on BRCA1 expression in breast and ovarian cancer cell lines.
BRCA1 expression was investigated in estrogen receptor (ER)-positive breast MCF-7 and ovarian BG-1 cell lines, and ER-negative breast MDA-MB-231 and HBL-100 cell lines. BRCA1 mRNA expression was measured by the ribonuclease protection assay and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). BRCA1 protein expression was determined using western blot analysis with an anti-BRCA1 antibody.
B[a]P exposure reduced BRCA1 transcripts in the ER-positive MCF-7 and BG-1 cell lines in a dose-dependent and time-dependent fashion, after both acute and chronic exposure. Loss of BRCA1 mRNA was paralleled by down regulation of BRCA1 protein. Exposure of the cells to the reactive metabolite of B[a]P,7r,8t-dihydroxy-9t,10t-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene (BPDE) also inhibited BRCA1 mRNA expression in ER-positive cells. A similar decrease in BRCA1 expression was not seen in the ER-negative cell lines MDA-MB-231 and HBL-100. Benzo[e]pyrene (B[e]P), which has a low affinity for the AhR, showed no influence on BRCA1 in MCF-7 cells. The halogenated aryl hydrocarbon 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which has a high affinity for the AhR, but is not metabolized, also had no effect on BRCA1 levels.
Previous reports have documented downregulation of BRCA1 mRNA in human breast and ovarian cancer cells by DNA-damaging and cytotoxic agents, including doxorubicin and ultraviolet radiation. Here, evidence is presented for a decrease in BRCA1 expression due to B[a]P and its reactive metabolite in ER-positive, but not ER-negative, breast and ovarian cancer cell lines. Involvement of the AhR pathway implicates the receptor as a negative transcription factor.