Brca1 and homologous recombination
- David Bertwistle
© Current Science Ltd 1999
Published: 1 December 1999
KeywordsDouble strand break gene targeting homologous recombination mouse embryonic stem cells non-homologous end joining
Mouse and human cells mutant for BRCA1 or BRCA2 are sensitive to DNA-damaging agents, implicating these genes in the response to DNA damage. Mammalian cells have two major pathways for the repair of DNA double-strand breaks (DSBs), homologous recombination and non-homologous end joining (NHEJ). The BRCA1 and BRCA2 proteins colocalise with RAD51, the mammalian equivalent of the yeast Rad51 protein, which is involved in the repair of DNA DSBs by homologous recombination. This has led to the view that BRCA1 and BRCA2 might also be involved in the repair of DNA DSBs by homologous recombination.
To determine whether mutation of the Brca1 gene impairs the repair of DNA DSBs by either homologous recombination or NHEJ.
Although a role for Brca1 in the repair of DNA DSBs by homologous recombination has been suggested by previous studies, this important paper is the first to directly demonstrate such a role.
Gene targeting. Analysis of DNA repair at a restriction endonuclease site following endonuclease digestion.
Gene targeting efficiencies in Brca1-/- embryonic stem (ES) cells were compared with those of Brca1+/- ES cells for two targeting vectors. A pim1-Q targeting vector with a promoterless hygromycin resistance gene was 23-fold less efficient in Brca1-/- ES cells. An Rb targeting vector with a PGK promoter driven hygromycin resistance gene was 53-fold less efficient in Brca1-/- ES cells. This 53-fold difference was due to a 13-fold decrease in gene targeting and a four fold increase in random integration.
DNA repair substrates containing the rare cutting endonuclease, I-SceI were integrated at the pim1 and Rb loci, in Brca1-/- and Brca1+/- ES cells. DSBs were introduced at the I-SceI site by electroporating cells with an I-SceI expression plasmid, and the resulting repair products were analysed using two assays. Compared to Brca1+/- cells, the Brca1-/- cells have a five to six fold lower level of homologous repair of DNA DSBs. No decrease in NHEJ was observed in Brca1-/- cells, rather it was increased by 1.5 to 1.6-fold.
The slight elevation in NHEJ in Brca1-/- cells, as measured by increased random integration of transfected DNA, may reflect an elevated level of spontaneous DSBs into which transfected DNA can insert. This could be due to the homologous repair defect in the Brca1-/- cells.
The Brca1 mutation in this study disrupts the terminal part of exon 10 and the beginning of exon 11. This does not result in a complete null allele, since an exon 10-12 splice variant is expressed. This variant does not contain exon 11, which encodes the RAD51 interacting domain as mapped in the human protein.
Impaired repair of DNA DSBs by homologous recombination would be predicted to result in genetic instability. Recent studies showing chromosomal abnormalities in Brca1-deficient mouse and human cells support a role for Brca1 in the maintenance of genetic stability.