Figure 2From: DACT1, an antagonist to Wnt/β-catenin signaling, suppresses tumor cell growth and is frequently silenced in breast cancerDACT1 was downregulated in primary breast tumors. (A) Analysis of DACT1 expression in normal breast tissues and primary breast tumors by using real-time PCR. (B) Representative immunohistochemical (IHC) staining for DACT1 in paired breast tumors and surgical-margin tissues. Magnification, 400×. (C) The mean optical density of DACT1 expression in each case (left panel). Quantitative analysis of DACT1 expression density is shown as values of mean ± SD (right panel). ***P < 0.001. (D, E) GSA-tumor analysis of DACT1 by using the 1,881-sample breast cancer data set. Box plot of DACT1 expression for tumor samples stratified according to Hu subtypes, ER status, and histologic grade.Back to article page