Fig. 6

Inhibition of EZH2-Integrin α11 suppresses growth and metastasis of CAM tumors with drug-resistant breast cancer

(A) Xenografted TAMR and ADR tumor growth and angiogenesis were inhibited by silencing of ITGA11, SUZ12, and EZH2, as well as EZH2 inhibitor GSK-126. The top panel shows photo image of tumors grown onto the CAM (dorsal, D) and angiogenesis on the ventral (V) sides of the cancer cell-inoculated membrane. The middle and bottom panels show the photographs of the tumor masses isolated from the xenografts and quantification of tumor weight, respectively. (B-D) Metastasis of tumor cells labeled with CMTPX, a cell-tracking red fluorescent dye, into liver and bottom side CAM tissue was inhibited by ITGA11/EZH2 axis inhibition. Compared to MCF-7 cells, TAMR and ADR cells exhibited more metastasis to the liver and opposite site (bottom) of the CAMs inoculated with cancer cells (B). The level of metastasis into liver and bottom CAM was quantified by qPCR for human housekeeping gene, HPRT. The effects of KD of ITGA11, SUZ12, and EZH2, and GSK126 treatment on metastasis to liver (C) and lower CAM (D) were quantified by human HPRT qPCR. *p < 0.05 compared to siNT-transfected group