Fig. 7

AMD1 promotes tumorigenicity in vitro and in vivo. (A, B) Soft-agar assay was performed using BT549 and Hs578T cells with or without AMD1 (A) as well as MDA-MB468 and SUM159 cells with stable empty vector or knockdown of AMD1 expression (B). Data are presented as the percentage of vector cell lines. Data are shown as mean ± SD based on three independent experiments. (C-E) MDA-MB468 and SUM159 cells with stable empty vector or knockdown of AMD1 expression were injected into the mammary fat pad of nude mice. Tumor growth was measured every four days. On day 26, mice were sacrificed and tumor weights were recorded. Tumor size (C) and weight (D) were measured and recorded. Expression of AMD1 was analyzed by Western blotting in tumor samples removed from two group mice (E). Data are presented as mean ± SEM of five mice. *p < 0.05 by Student’s t test. (F) Box-plots indicated AMD1 expression in different histological grades of breast cancer from GSE25066, NKI295, MEBTABRIC and GSE7390 datasets. Comparisons between two groups are made using the two-tailed Student’s t-test