Fig. 6

Innate- and T-cell-related gene expression levels corroborate the immunophenotypic changes upon chitin treatment in 4T1 and 66cl4 primary tumors. A-D Heatmaps showing mean normalized expression levels of selected genes associated with innate immunity in 4T1 (A) and 66cl4 primary tumors (B), T-cell exhaustion in 4T1 primary tumors (C) and T-cell activation in 66cl4 primary tumors (D) at 5 w p.i. derived from untreated, anti-PD-1, chitin- and chitin + anti-PD-1-treated tumor-bearing mice. Mean normalized expression levels were calculated based on normalized expression levels in 4 or 5 primary tumor samples from each treatment group and for each model. Selection of the genes was based on gene lists from the NanoString Mouse PanCancer Immune Profiling Panel. Pearson distance was used for hierarchical clustering