Fig. 2
Chitin and anti-PD-1 combination has a reductive effect on metastatic progression in the 4T1- and 66cl4-based intraductal model. A,B Representative images and quantification of bioluminescent signals (total flux density in p/s/cm2) in lungs from the untreated and treated 4T1- (A) and 66cl4-based model (B) at 5 w p.i. (n = 7 for all groups in the 4T1-based model; n = 11 for all groups in the 66cl4-based model). C H&E histology of lung metastases from the untreated and treated 4T1- and 66cl4-based model at 5 w p.i. Dashed inserts highlight H&E-stained metastases at a larger magnification. Black scale bars = 200 µm, red scale bars = 50 μm. D,E Representative images and weight measurements of the spleen from the untreated and treated 4T1-(D) and 66cl4-based model (E) at 5 w p.i. (n = 5 for all groups in the 4T1-based model; n = 11 for all groups in the 66cl4-based model). An image of a healthy spleen is shown for comparison and the dotted line in the graph highlights the mean spleen weight of 4 healthy BALB/c mice. Data are presented as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001