Fig. 3

PTHrP lacking the NLS and C-terminal domain regulates proliferation by altering expression of p27. (A) Number of genes identified by RNAseq with log₂fold change > 1 and p < 0.05. (B) GSEA plot from DNLS cells showing enrichment of Cyclin D1 gene signature in MCF7 cells. (C) GSEA plot from DNLS + CTERM cells showing enrichment of genes that regulate the G2M checkpoint. (D) Top twenty enriched Hallmark pathways from FLSEC, DNLS, and DNLS + CTERM cells. (E) Immunocytochemical staining and quantification of p27 in MSCV, FLSEC, DNLS, or DNLS + CTERM cells. n = 3 independent biological replicates. All panels = 40X, scale bar = 25 μm. (F) Immunofluorescence staining and quantification for p27 in primary tumors from mice inoculated with MSCV, FLSEC, DNLS, or DNLS + CTERM cells. All panels = 40X, scale bar = 50 μm. (E) **p < 0.01 or ****p < 0.0001 vs. MSCV by one-way ANOVA with multiple comparisons or ****p < 0.0001 vs. DNLS by unpaired t-test. (F) *p < 0.05 or ***p < 0.001 vs. MSCV by one-way ANOVA with multiple comparisons or **p < 0.01 vs. DNLS by unpaired t-test. Graphs represent mean ± SEM