Fig. 2

Deletion of the PTHrP NLS alters breast cancer cell proliferation and primary tumor growth. (A) Time to tumor palpation, (B) tumor volume over time by digital caliper measurement and (C) final tumor weight in mice inoculated with MSCV, FLSEC, DNLS, or DNLS + CTERM cells into the mammary fat pad. n = 7–10 mice/group. (D) Ki67 staining and quantification from tumors in (A-C). (E) Quantification of mitoses (# mitotic figures/total cells in 40X field) by DAPI staining from tumors in (A-C). (F) Cleaved PARP staining and quantification from tumors in (A-C). All panels = 40X and scale bar = 50 μm. (A) *p < 0.05 vs. MSCV by one-way ANOVA with multiple comparisons or #p < 0.05 DNLS vs. DNLS + CTERM by unpaired t-test. (B) ****p < 0.0001 vs. MSCV by one-way ANOVA with multiple comparisons or **p < 0.01 vs. DNLS by unpaired t-test. (C) **p < 0.01 vs. MSCV by one-way ANOVA with multiple comparisons or ***p < 0.001 vs. DNLS by unpaired t-test. (D) **p < 0.01 vs. MSCV by one-way ANOVA with multiple comparisons. (E) *p < 0.05 vs. MSCV by one-way ANOVA with multiple comparisons or *p < 0.05 vs. DNLS by unpaired t-test. Graphs represent mean ± SEM