Fig. 7

Matrices with EDA+ fibronectin induce the assemblage of α-SMA fibers in naïve fibroblasts. A MSC or NIH3T3 fibroblasts are induced to assemble α-SMA fibers by EDA+ matrices. MSC or NIH3T3 were grown 24 h on decellularized 3D ECMs generated by EDA– or EDA+ MEFs before detecting α-SMA and nuclei by IF. Images obtained through fluorescence microscopy were used to quantify the percentage of cells presenting α-SMA positive stress fibers. B NIH3T3 fibroblasts are induced to assemble α-SMA fibers by control and EDA matrices. NIH3T3 fibroblasts were grown 24 h on decellularized 3D ECMs generated by the indicated MEFs in the presence or absence of 5 ng/mL TGFβ₁. The percentage of fibroblasts presenting α-SMA positive stress fibers was quantified as in A. C Irigenin interferes with fibroblast activation by EDA+ matrices. NIH3T3 fibroblasts were grown for 24 h on decellularized 3D ECMs generated by the indicated MEFs in the presence or absence of 5 ng/mL TGFβ₁, and the presence or absence of 50 μM irigenin. The percentage of fibroblasts presenting α-SMA positive stress fibers was quantified as in A