Fig. 2

Heterogeneous and conserved somatic features revealed by whole genome sequencing. Representative Circos plots are shown for (A) microacinar, (B) squamous, and (C) EMT histological subtypes. The outermost ring of each Circos plot depicts an ideogram for the mouse chromosomes proportionate with actual chromosome length. The next inner ring shows mutations in genes as stacked blocks at their corresponding genomic locations, color coded to their predicted impact by SnpEff [40]—yellow for low impact, orange for moderate impact, and red for high impact. The next inner ring shows discrete copy number changes as analyzed by CNVKIT; red regions indicate amplification and blue regions indicate deletions. The height of each copy number alteration corresponds to the predicted change in copy number, with the lowest level change being \(\pm \hspace{0.17em}1\) and showing a max copy number change of \(\pm\) 2. The innermost ring reveals inversions and translocations as determined by the consensus of Delly and Lumpy somatic variant callers. Inversions are colored black, while translocations match the color of the ideogram of one of the two chromosomes involved in the translocation event. (D) A CNVKIT heatmap shows the log₂ fold change of the estimated normalized copy number segments of each chromosome for each tumor sample relative to the wildtype reference