Fig. 5

B7-H4 deficiency promotes breast cancer cell metastasis in vivo. A, B A commonly employed murine model to study breast cancer lung metastasis entails the injection of cancer cells via the tail vein to implant cells in the lung, and lung metastasis tissues were isolated 4 weeks after implantation. Scale bar = 5 mm. The representative lung tissue images and HE staining (Scale bar = 100 μm) of A SKBR3 (WT and KO) and B MDA-MB-231 (WT and OE) were shown. The black arrows represent the location of the metastatic tumors. C The number of metastatic nodules in representative lung tissue of each group was calculated. D The total number of mice with and without metastatic lung tumors (metastasis and non-metastasis) in each group (n = 5) was calculated. E, F The expression levels of (E) E-Cad and (F) N-Cad in SKBR3-WT and SKBR3-KO inoculated mouse lung tissues were investigated by immunofluorescence. Scale bar = 100 μm. E-Cad: E-Cadherin; N-Cad: N-Cadherin. Data are represented as mean ± SEM of three independent experiments, and statistical significance was determined by two-tailed unpaired t test. (***p < 0.001)