Fig. 2

B7-H4 interferes human breast cancer cell proliferation and migration. A The mRNA expression levels of B7-H4, hormone receptors (ER and PR) and HER2 in different types of breast cancer cell lines were analyzed using CCLE database. B The protein expression levels of B7-H4 were analyzed by immunoblotting in various human breast cancer cell lines (left graph) and the engineering breast cancer cells with B7-H4 knockout or B7-H4 overexpression (right graph). C–E Cell invasion ability was compared between wide type and engineering tumor cell lines including SKBR3, MDA-MB-231 and MCF7, using transwell matrigel invasion assay. F–H A wound healing assay detected cell migration analysis in paired tumor cell lines. Scale bar = 100 μm. Representative images and quantified data were shown in the graphs. Data are represented as mean ± SEM of three independent experiments, and statistical significance was determined by a two-tailed unpaired t test (*p < 0.05, ***p < 0.001). CCLE: Cancer Cell Line Encyclopedia; WT: wide type cell lines; KO: B7-H4 knockout cell lines; OE: B7-H4 overexpression cell lines