Fig. 5

B-cell infiltrates are associated with unfavorable outcomes. A is a close-up dot plot of Fig. 2 focused on all significant associations between B-cell related expression with either overall survival or radiological complete remission in at least one analysis. Blue dots indicate increased overall survival or higher likelihood of radiological complete response. Red dots indicate decreased overall survival or lower likelihood of radiological complete response. The size of the dot is proportional to the P value with larger dots indicating a smaller P value. The background color is white for P < 0.05, light gray for P > 0.05 and < 0.10 and gray for P ≥ 0.10. P values shown are not adjusted for multiple testing. B is a multiplex immunofluorescence image showing B-cells (CD20 + ; magneta-colored cells) and T-cells (CD3 + ; green-colored cells) as well as tumor cells (CK + , yellow-colored cells). C shows infiltration of B-cells in primary tumors in patients with radiological complete response (blue) and patients with no radiological complete response (yellow). D shows the overall survival probability according to B-cells and T-cells colocalization intensity measured with the Morisita-Horn index in primary tumors split in tertiles. E shows the overall survival probability according to expression of the B-cell metagene signature in primary tumors split in tertiles