Fig. 4

Infiltration of Tregs and exhausted T-cells are associated with unfavorable outcomes. A is a close-up dot plot of Fig. 2 focused on all significant associations between T-cell related expression with either overall survival or radiological complete remission in at least one analysis. Blue dots indicate increased overall survival or higher likelihood of radiological complete response. Red dots indicate decreased overall survival or lower likelihood of radiological complete response. The size of the dot is proportional to the P value with larger dots indicating a smaller P value. The background color is white for P < 0.05, light gray for P > 0.05 and < 0.10 and gray for P ≥ 0.10. P values shown are not adjusted for multiple testing. B is a multiplex immunofluorescence image showing FoxP3-positive T cells (Tregs; red-colored cells) and FoxP3-negative T cells (green-colored cells), indicated by red arrows. C shows the overall survival probability according to infiltration of regulatory T-cells (Tregs) split in tertiles. D shows the overall survival probability according to the exhausted T-cell signature split in tertiles. E is a multiplex immunofluorescence image showing tumor cells (yellow cells), PDL1-positive tumor cells (red cells) and a merged image of PDL1-positive tumor cells. F shows PDL1 expression (low vs high) in ER- primary tumors in radiological complete response (pink) and no radiological complete response (yellow). G shows PDL1 expression (low vs high) in ER-negative metastases in radiological complete response (pink) and no radiological complete response (yellow)