Fig. 3

Treatment with ZBC260 decreases markers of stemness and increases differentiation. a Representative flow cytometry graphs of ALDH, CD44, and CD24 in SUM149 cells. b–d Percentage of b ALDH⁺ cells, c CD44⁺ /CD24⁻ cells, and d CD24⁺ /CD44⁺ cells in SUM149 cell line treated at varying concentrations of ZBC260. e Representative flow cytometry graphs of ALDH, CD44, and CD24 in SUM159 cells. f–h Percentage of (f) ALDH⁺ cells, g CD44⁺ /CD24⁻ cells, and (h) CD24⁺ /CD44⁺ cells in SUM159 cell line treated at varying concentrations of ZBC260. i–j Primary and Secondary sphere formation counts of SUM149 and SUM159 cells treated with ZBC260 at varying concentrations. k Size comparison of SUM159 Primary and Secondary tumorspheres. Data points are the mean ± SEM for individual experiments n = 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001