Fig. 2

ZBC260 significantly inhibits tumor growth in vivo. a Cell viability of SUM149, SUM159, MDA-MB-453, and MDA-MB-468 cells treated with ZBC260 b Comparison of JQ1 vs ZBC260 on cell viability in SUM149 and SUM159 cells. The results presented are representative graphs of at least 3 biological replicates. Data points are the mean ± SEM for individual experiments. c Colony-formation efficiency, reported as survival fraction, of SUM149 and SUM159 cells treated with ZBC260. Mean ± SEM for n = 3 independent experiments. d Representative images of colony formation assay of SUM149 and SUM159 cells treated with 0 nM, 12.5 nM, or 25 nM ZBC260. e Effect of ZBC260 or vehicle on SUM149 tumor volume in C.B.17SCID mice over 42 days of treatment. f The tumor initiation frequency calculated by ELDA of reimplanted tumors after ZBC260 or vehicle treatment. The results presented are representative graphs of at least 3 biological replicates. Data points are the mean ± SEM for individual experiments, n = 5 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.00; ****P < 0.0001