Fig. 8
From: Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors
Expression and function of BMPR1B gene in epithelial cells. a Volcano plots of BMPR1B gene expression in cluster 12 from different zones here (left to right): Tumor zone versus interface zone, interface zone versus normal zone and tumor zone versus normal zone. b Heatmap of the average expression trend of BMPR1B gene in luminal cells across the three zones (normal, interface and tumor). c Box plots showing the log2(TPM + 1) value of BMPR1B gene in basal-like breast cancer (TCGA datasets, n = 135), HER2 + breast cancer (TCGA datasets, n = 66), luminal A breast cancer (TCGA datasets, n = 415), luminal B breast cancer (TCGA datasets, n = 194) and normal breast tissues (TCGA and GTEx data, n = 291), **p < 0.01. d BMPR1B overexpression in different types of epithelial cells was determined by RT-qPCR analysis. Matched cell lines with raw vector were used as control group, **p < 0.01 vs. control. e Line chart of cell proliferation measured at 450 nm, **p < 0.01. f Wound-healing assay of MCF10A and MCF-7 cells with or without BMPR1B overexpression. Left: Representative images of BMPR1B-overexpressed cells and control at three time point after wounding. Right: quantification of the reduction rate of scratch area (mean ± SD). g Photographs of cell clones observed under a microscope. BMPR1B gene promoted the colony forming ability of MCF7 cells. h Photographs of BALB/cNj-Foxn1nu/Gpt mice. BMPR1B-overexpressed MCF-7 cells and control cells were injected into subcutaneous location of left and right groin in nude mice, respectively. 15th day post-transplantation, mice were photographed, and tumors were removed surgically and weighed. i Statistical analysis of mice tumor weights (n = 3, *p < 0.05, t test)