Fig. 6
From: Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors
Fibroblast clusters. a tSNE plots of 7,100 fibroblasts, color by relevant clusters (top) or sample type (bottom). b Color coding for expression (gray to blue) of marker genes of fibroblasts. c Boxplots showing signature score of marker genes. The signature score is calculated by the average expression of log2(TPM + 1) of each gene in top 10 marker genes for cluster 4, 7 or 10 from breast cancer tissues (TCGA datasets, n = 1085) and normal breast tissues (TCGA and GTEx data, n = 291), **p < 0.01. d Target candidate staining of tumor, interface and normal zone. Nucleus stained by DAPI (blue), fibroblasts stained by DCN (green) and marker of interest depicted by MMP11 (red). DCN, decorin. MMP11, matrix metallopeptidase 11. Representative images were displayed. e Bubble plots displaying the expression of specific genes in fibroblast clusters. f Differences in pathway activity of each cell scored by GSVA between fibroblast clusters. g Violin plots displaying expressions of the selected genes in fibroblast clusters