Fig. 6

miR-203 transitory exposure induces a basal-to-luminal shift on mouse mammary tumor-derived organoids. A Schematic showing the correlation between cytokeratins expression, histopathological tumor grade, prognosis and breast cancer type. B Left panel, Illustrative detection of CK8/18, CK14 and CK5 by IHC in control tumors and miR-203-treated tumors at the experimental endpoint. Right panel, Violin plots showing the quantification of markers staining. The doxycycline schedule followed for this set of experiments is also the one indicated in Fig. 3A. C Left panel, Illustrative IHC images of staining for CK8/18, CK14 and CK5 in control tumor-derived organoids, control tumor-derived organoids treated in vitro with miR-203 during 5 days and healthy mammary gland-derived organoids. Right panel, Violin plots showing the quantification of markers staining. D Left panel, Detection of CK8/18 (red), CK14 (green) and E-cadherin (purple) by immunofluorescence in tumor-derived organoids, extracted from control tumors, miR-203-treated tumors or healthy mammary gland tissue samples. Right panel, Violin plots showing the quantification of markers staining. In B, C scale bar, 500 µm; in D: scale bar, 100 µm. In violin plots, six different fields from three independent tumor samples were analyzed. ****p < 0.0001; *p < 0.05; n.s. not statistically different (Student’s t test for panel B; One-way ANOVA for panels C, D)