Fig. 7
From: RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer
RAGE inhibition prevents Ins-induced cell proliferation and glucose utilization in MCF-7 cells. Evaluation of cell proliferation by cell counting in MCF-7 (A) and ZR75 B cells treated with Ins (20 nM, 72 h), alone and in combination with FPS-ZM1 (10 μM), or RAP (50 μM) (C). Evaluation of cell proliferation in MCF-7 cells transfected with siRAGE (10 nM) or non-targeting scramble siRNA for 24 h before stimulation with Ins (20 nM, 72 h) (D). Efficacy of RAGE silencing as evaluated by Western blotting (E). Representative tracing of Extracellular acidification rate (ECAR) in MCF-7 cells treated with Ins (20 nM, 8 h) alone and in combination with FPS-ZM1 (10 μM, 24 h) (F). Evaluation of glycolysis, glycolytic reserve and glycolytic reserve capacity (G). Data shown are the mean ± SEM of at least two independent experiments. (*) p < 0.05; (**) p < 0.01; (***) p < 0.001