Fig. 5
From: RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer
Proteomic analysis in MCF-7 cells. Schematic representation of experimental workflow for unbiased label-free proteomics study performed in MCF-7 cells exposed to Ins (20 nM, 4 h), alone and in combination with FPS-ZM1 (10 μM, 24 h) (A). Venn diagram showing the overlap of proteins differentially expressed (DEPs) across all treatments compared to vehicle-treated cells (B). Volcano plots showing the identified DEPs in cells stimulated with treatments compared to vehicle-treated cells, as indicated (C, E). X axis is the fold change (FC) (log2) of protein expression versus vehicle-treated cells, and the Y axis represents p (−log10). Red points (FC > 0.6) indicate up-regulated proteins; blue points (FC < 0.6) indicate down-regulated proteins, and gray points indicate proteins without significant differential expression. Bottom panels report the top-10 up-regulated (in red) and down-regulated (in blue) proteins in experimental groups