Fig. 4

FPS-ZM1 halts IR and IGF-1R-mediated signals. The Ins-induced activation of IR/IGF-1R (Y1135/1136) (A), IRS1 (Y612) (B) and AKT (S473) (C), as well as the up-regulation of CD1 D is attenuated by FPS-ZM1 in MCF-7 cells engineered for the deletion of IR (KO-IR) (E). FPS-ZM1 interferes with the Ins-induced activation of IR/IGF-1R (Y1135/1136) (F), IRS1 (Y612) (G) and AKT (S473) (H), as well as the up-regulation of CD1 I in MCF-7 cells engineered for the deletion of IGF-1R (KOIGF-1R) Representative immunoblots showing the protein expression of pIR/pIGF-1R (Y1135/1136), pIRS1 (Y612), pAKT (S473) in MCF-7 cells treated for 15 min with IGF-1 (10 nM) (I−K) or IGF-2 (10 nM) (M, O), alone and in combination with FPS-ZM1 (10 μM, 24 h). Representative immunoblots showing the protein expression of CD1 in MCF-7 cells treated for 4 h with IGF-1 (10 nM) (L) or IGF-2 (10 nM) (P), alone and in combination with FPS-ZM1 (10 μM, 24 h). Total proteins and β-actin serve as loading control. Data shown are the mean ± SEM of at least three independent experiments. (*) p < 0.05; (**) p < 0.01; (***) p < 0.001