Fig. 10

FPS-ZM1 interferes with Ins-induced growth of 4T1-derived allografts. Schematic representation of the experimental workflow for the in vivo studies. Female athymic nude mice were inoculated with 4T1 cells. On the seventh day after inoculation, mice were treated with 10 nM Ins glargine, given s.c. for 5 days/week, alone and in combination with FPS-ZM1 (1 mg/kg) given i.p., twice a week (n = 6 for each group). At day 28, mice were sacrificed and tumor tissue was collected (A). Graph showing the tumor volumes (cm3) at indicated time points from the starting of treatments. Representative images of explanted tumors are shown. The data are the mean ± SEM of the values obtained in six animals per group. (*) p < 0.05 (B). Tumors’ weight in mice at sacrifice (C). Evaluation of blood glucose levels in mice, as indicated (D). Representative immunoblot showing the evaluation of CD1 protein expression in tumor homogenates from 4T1-derived allografts. β-actin serves as loading control (E). Data shown are mean ± SEM of at least three independent experiments. (*) p < 0.05