Fig. 8

miR-26a-5p is a transcriptional downstream target of ER-a and PR. A bc-GenExMiner was used to analyze the expression level of miR-26a-5p which was positively correlated with ER-a status and PR status, not HER2 receptor status. B After hormone deprivation for 48 h, MCF-7 cells were stimulated with β-Estradiol (E2). RT-qPCR was used to monitor the expression of miR-26a-5p in different concentration or at different time points. C After hormone deprivation for 48 h, MCF-7 cells were stimulated with Etonogestrel (ETO). RT-qPCR was used to monitor the expression of miR-26a-5p in different concentration or at different time points. D, E Overexpressed ER-a or PR plasmid were transfected into MDA-MB-231 cells. RT-qPCR was used to detect the expression of miR-26a-5p in normal culture conditions without or with extra hormone stimulating. F JASPAR and hTFtarget databases were used to predict the potential binding sites of ER-a and PR with the promoter of miR-26a-5p. G, H ChIP assay was used to verify the binding sites. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus control. n = 3