Fig. 7

Implication of myocardin-related transcription factor (MRTF) during luminal apoptosis in normal and accelerated formation of acini. a Cells were grown in matrigel with 10% collagen for the last 2 days if indicated. Phalloidin (red), cleaved caspase3 (green), Ki67 (red) and DNA (blue) staining of representative mid plane sections is shown. Quantification of Ki67-positive cells per acini; 30 acini each were analyzed. Scale bar 20 μm. b Quantification of lumen formation of mock-transfected control acini at day 8. Analysis of 30 acini each, with or without prior collagen treatment. c Time course of MRTF/serum response factor luciferase reporter (SRF) activity during 3D morphogenesis of stably transfected MCF10A reporter cells, normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein abundance. Error bars SEM (n = 3): *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test)