Fig. 4

Spleens from late-stage collagen-dense tumor mice are enlarged relative to wild-type (WT) mice. a Example spleens from WT (left) and tumor mice expressing the polyoma virus antigen and heterozygote for the col1a1 mutation (COL) (right). Note the increased size of the COL spleens. b Spleen weight (mg) normalized to mouse weight (g) from both WT and COL mice (n = 7). c Percentage of CD45+ immune cells, d Ly6G+Ly6C+ neutrophils and e F4/80+ macrophages found in WT and COL spleens, determined by flow cytometry as in Fig. 3. Note that there is a trend toward decreased CD45+ immune cells (p = 0.13) and increased Ly6G+Ly6C+ neutrophils in COL tumors (p = 0.18) (f) CD45+ immune cells (lymphoid panel, fixed cells), (g) CD3+ T-cells, (h) CD8+ cytotoxic T-cells, (i) CD4+ T helper cells, and (j) Foxp3+ T regulatory cells found in WT and COL spleens, determined by flow cytometry as in Fig. 2)