Fig. 4From: Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cellsGene expression of cell isolates. Real time PCR results show gene expression (n = 48) genes, including a house keeping gene after comparison to the mean expression of all normal (NORMA1-4) respective MEC, MES or ADSC primary cell lines. a Expression of positive stem cell markers CD105, CD73, CD90, CD36, CD29, CD44 and of vimentin (VIM) (ADSC=1) and hematopoietic markers CD14, CD19, CD34, CD45, HLA-DR are shown (PBC/BEC=1). b Panel shows invasion and EMT marker genes, MMP2, MMP9 and SNAI1, the fibroblast mesenchymal marker S100A4 and N-cadherin (CDH2) and CDH11, markers of the mesenchymal phenotype (MES=1). c Epithelial cell markers (MEC=1) compared to ADSC and MES. Specific markers include epithelial stem cell markers CD24 and CD49f and markers of basal myoepithelial mammary cells (ACTA1, CNN1, ACTA2, CD10). d Six endothelial marker genes analyzed were PECAM1, VWF, FLT1, KDR, FLT4, CDH5 and PDGFRB (BEC=1). Significant values compared with BEC (*, **) or MEC NORMA1 (#, ##). (*,# p ≤ 0.05, **,## p ≤ 0.01) (ADSC = adipose derived stem cells, BEC = blood endothelial cells MEC = mammary epithelial cells, MES = mesenchymal cells, PBC = peripheral blood cells)Back to article page