Figure 3

Osteopontin (OPN) gene expression in vitro. TM6 cells were starved for 24 hours before stimulation for 1 hour with growth factors (GF). A ninefold increase was noted in OPN expression within 1 hour of stimulation compared with the OPN level in starved cells. When the starved cells were treated with Se-methylselenocysteine (MSC) for either 1 hour or 24 hours before stimulation with GF, the expression of OPN was decreased 0.2-fold and 4.4-fold respectively compared with the 1-hour-stimulated OPN level in the starved cells. The fold change was normalized with β-actin levels in the corresponding lanes.