Figure 3
From: The Icelandic founder mutation BRCA2 999del5: analysis of expression
No mutant protein could be detected by ELISA. (a) Lysates of fibroblasts with wild-type BRCA2 (wt) and fibroblasts heterozygous for BRCA2 999del5 (Mut) were tested for the presence of BRCA2 999del5 protein: bar pairs 3 and 4, lysates diluted 1:10; bar pairs 5 and 6, lysates diluted 1:100; bar pairs 7 and 8, lysates diluted 1:1000. (b) The same experiment was performed using lysates from untransfected COS7 cells (COS7), and COS7 cells overexpressing pIND-LacZ (Mock) or pIND-BRCA2 999del5 (Mut): bar pairs 3, 4 and 5, lysates diluted 1:10; bar pairs 6, 7 and 8, lysates diluted 1:100; bar pairs 9, 10 and 11, lysates diluted 1:1000. The synthetic peptide, representing the 16 out-of-frame amino acids (a.a.) of BRCA2 999del5 and used to raise the antiserum, was used as a positive control.