From: Homeobox genes in mammary gland development and neoplasia
Gene | Gene | Expression | Â | Expression altered in | Â | Mammary or in vitro | Â |
---|---|---|---|---|---|---|---|
family | namea | detected inb: | Methodc | neoplasias ? | Possible regulation? | phenotype? | Reference |
Hox/HOX | Hoxa-1 | MG (UD) | RT-PCR; Northern | OE (freq.) | Extracellular matrix | Â | |
 |  | Scp2, CID-9 |  |  | components |  |  |
 | HOXA-1 | MCF7 | RT-PCR |  | Retinoic acid |  | |
 | Hoxa-5 | CID-9 | RT-PCR |  |  |  | [33*] |
 | Hoxa-7 | MG | RT-PCR |  |  |  | [31*] |
 | HOXA-7 | MG | RT-PCR |  |  |  | 1 |
 | Hoxa-9 | MG | RT-PCR |  |  | KO: lactational defects | [38*] |
 | HOXA-10 | MCF7 | RT-PCR |  | Vitamin D | OE in MCF7: promotes cell cycle | |
 |  |  |  |  |  | arrest at G1 |  |
 | Hoxb-6 | MG | RT-PCR; Northern | Lost |  |  | [31*] |
 | HOXB-6 | MCF7 | RT-PCR |  |  |  | [30] |
 | Hoxb-7 | MG (E+S) | Northern; in situ | UE | Extracellular matrix | OE in SkBr3 cells: bFGF induction; | |
 |  |  |  |  | components; developmental | increased proliferation; decreased |  |
 |  |  |  |  |  | growth factor dependency |  |
 | Hoxb-8 | CID-9 | RT-PCR |  |  |  | [33*] |
 | Hoxb-9 | CID-9 E12.5 | RT-PCR; in situ |  |  | KO: lactational defects | |
 |  | Embryo (MM) |  |  |  |  |  |
 | Hoxc-6 | MG | Northern | Lost | Estrogen (-); developmental |  | [31] |
 | HOXC-6 | MCF7; MCF7D; | cDNA screen; |  | Variable |  | |
 |  | Hs578Bst; | RT-PCR |  |  |  |  |
 |  | MCF10F; T47D |  |  |  |  |  |
 | Hoxc-8 | MG | Northern | Lost | Estrogen (-); developmental |  | [32] |
 | HOXC-10 | MG | PCR of cDNA library |  |  |  | 2 |
 | Hoxd-3 | MG (UD) | Northern | OE (rarely) |  |  | [32] |
 | Hoxd-4 | MG (E) | In situ | Lost |  |  | [32] |
 | Hoxd-8 | MG | Northern | Lost | Developmental |  | [32] |
 | Hoxd-9 | MG (E+S); | RT-PCR; | Lost | Developmental | KO: lactational defects | |
 |  | E12.5 Embryo | Northern; in situ |  |  |  |  |
 |  | (MM) |  |  |  |  |  |
 | Hoxd-10 | MG (E+S) | Northern; in situ | Lost | Developmental | KO: lactational defects | |
 | Hoxd-11 | MG (UD) | Northern | No |  |  | [32] |
 | Hord-12 | MG | RT-PCR; | OE (myc tumor) |  |  | [31*] |
 |  |  | Northern (UD) |  |  |  |  |
Msx | Msx-1 | E13.5 Embryo (E); | Northern; in situ | Maintained but variable | Developmental | KO: none detected in embryos | |
 |  | MG (E) |  |  |  |  |  |
 | Msx-2 | E13.5 Embryo (E); | Northern; in situ | Lost | Estrogen; developmental | KO: MG fail to form | [40*];3 |
 |  | MG (S) |  |  |  |  |  |
Iroquois related | IRX-1 | MG | RT-PCR | Â | Â | Â | [48*] |
 | IRx-2 | MG; primary | RT-PCR; Northern; | Maintained, expression | Developmental |  | [48*] |
 |  | tumors | in situ | levels or splice form |  |  |  |
 |  |  |  | usage may be altered |  |  |  |
 |  |  |  | in some tumors |  |  |  |
 | IRX-3 | MG | RT-PCR |  |  |  | [48*] |
 | IRX-4 | MG | RT-PCR |  |  |  | [48*] |
 | IRX-5 | MG | RT-PCR |  |  |  | [48*] |
Paired domain | Alx-4 | MG(S) | In situ | Â | Expression associated with | KO: none detected | [45] |
 |  |  |  |  | actively condensing periductal |  |  |
 |  |  |  |  | stroma |  |  |
HSIX | HSIX-1 | MCF7 | Â | OE | Expressed in S phase | OE in MCF7 abolishes X-ray induced | [58*] |
 |  |  |  |  | of cell cycle | cell cycle arrest in G2 phase |  |
POU domain | OCT-1 | MCF7; MCF10; | RT-PCR; | Â | Â | Â | [54] |
 |  | SkBr3;MDA- | Western EMSA |  |  |  |  |
 |  | MB453 |  |  |  |  |  |
 | Oct-1 | MG (lact.) | RT-PCR |  |  |  | [53] |
 | OCT-2 | MCF7; MCF10; |  |  |  |  |  |
 |  | SkBr3 | RT-PCR; |  |  |  | [54] |
 |  |  | Western EMSA |  |  |  |  |
 | OCT-3 | MG (UD); MCF7; | RT-PCR | OE (freq.) |  | Form with a 5 amino acid deletion | [54] |
 |  | MCF10; SkBr3; |  |  |  | detected om MCF7 cell |  |
 |  | primary tumors |  |  |  |  |  |
 | Oct-3 | MG (lact.) | RT-PCR |  |  |  | [53] |
 | OCT-11 | MG (UD) MCF7; | RT-PCR; EMSA | OE (freq.) |  |  | [54] |
 |  | skBr3; MDA- |  |  |  |  |  |
 |  | MB453; primary |  |  |  |  |  |
 |  | tumors |  |  |  |  |  |
 | Pit-rs 1 | MG (lact.) | RT-PCR |  |  |  | [53] |
 | Pou4f1 = Brn-3 | MG (lact.) | RT-PCR |  |  |  | [53] |
 | Pou4f-rs1 = | MG (lact.) | RT-PCR |  |  |  | [53] |
 | Brn-3R |  |  |  |  |  |  |
 | Pou3f4 = Brn-4 | MG (lact.) | RT-PCR |  |  |  | [53] |
 | Pou3f-rs1 = | MG (lact.) | RT-PCR |  |  |  | [53] |
 | POU-IIIA |  |  |  |  |  |  |
Engrailed related | En-1 | MG | Northern | UE (freq.) | Developmental | Â | [32] |
 | En-2 | MG (UD) | Northern | UD |  | KO: none detechted | [32] |
MOX | MOX1 | MG | Â | Â | Â | Â | [52] |
MEIS | MEIS2a | MG | RT-PCR of cDNA library | Â | Â | 4 | |
 | MEIS 2d | MG | RT-PCR of cDNA library |  |  | 4 | |
 | MRG1a | MG | RT-PCR of cDNA library |  |  | 4 |