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Figure 5 | Breast Cancer Research

Figure 5

From: Ubiquitin-conjugating enzyme complex Uev1A-Ubc13 promotes breast cancer metastasis through nuclear factor-кB mediated matrix metalloproteinase-1 gene regulation

Figure 5

Ubiquitin conjugating enzyme variant (Uev)1A positively regulates MMP1 and MMP9 expression. (A) The transcript levels of selected putative NF-κB target genes in MDA-MB-231-TR cells expressing different UEVs as determined by qRT-PCR. (B) Overexpression of UEV1A but not UEV1A-F38E stimulated MMP1 and MMP9 expression in MDA-MB-231 cells as determined by qRT-PCR. (C) Elevated MMP1 and MMP9 protein levels in UEV1A-overexpressed MDA-MB-231 cells as determined by western blot. (D) The transcript levels of MMP1 and MMP9 in two independent shUEV1-transfected MDA-MB-231 cell lines as determined by qRT-PCR. (E) MMP1 and MMP9 protein levels in two independent shUEV1-transfected MDA-MB-231 cell lines as determined by western blot.

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Breast Cancer Research

ISSN: 1465-542X

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