Figure 8

Diminution of lung and bone metastasis by interrupting the SCF/cKit signaling. (A) Significant decrease in tumor burden in the mice treated with anti-cKit (*P < 0.05). (B) Percentage incidence of lung metastasis by blocking the SCF/cKit signaling. (C) Representative images of 4T1-GFP cells in lungs of arthritic mice with no treatment. (D, E) Representative images of (D) H&E (200×) and (E) pancytokeratin (400×) staining of lungs to confirm micrometastasis. (F) Percentage incidence of bone metastasis by blocking the SCF/cKit signaling. (G, H) Representative images of pancytokeratin staining of (G) BM (200×) and (H) bone tissue (200×) to confirm metastasis. (I) Representative r-ray images of metastatic bone lesions in arthritic model with no treatment. (A through I, 10 mice.) Brown staining represents pancytokeratin positivity.