Table 1 Associations between CCND1 amplification status, cyclin D1 protein expression and clinicopathological variables in all patients
All patients | Cyclin D1 cytoplasmic intensity (negative/low, intermediate, high) | Cyclin D1 nuclear intensity (negative/low, intermediate, high) | Cyclin D1 nuclear fraction (< 1%,1 to 9%, 10 to 32%, 33 to 67%, > 67%) | Tumour grade (well, moderate, poor) | Tumour sizea (mm) | Nodal status (negative, 1 to 3, > 3) | Ki67a |
|---|---|---|---|---|---|---|---|
CCND1 amplification | |||||||
Nonamplified | 12%, 52%, 36% | 40%, 36%, 24% | 10%, 24%, 27%, 24%, 15% | 23%, 61%, 16% | 18.0 | 70%, 23%, 7% | 4.0 |
Amplified | 6%, 48%, 46% | 9%, 35%, 56% | 0%, 6%, 18%, 32%, 44% | 9%, 62%, 29% | 19.0 | 64%, 26%, 10% | 7.5 |
Armitage (P = 0.063) | Armitage (P < 0.001) | Armitage (P < 0.001) | Armitage (P < 0.001) | Wilcoxon (P = 0.083) | Armitage (P = 0.341) | Wilcoxon (P < 0.001) | |
Cyclin D1 cytoplasmic intensity | |||||||
Negative/low | 74%, 16%, 10% | 23%, 33%, 26%, 11%, 4% | 26%, 59%, 15% | 17.5 | 68%, 24%, 8% | 3.2 | |
Intermediate | 40%, 36%, 24% | 10%, 24%, 27%, 25%, 14% | 17%, 63%, 20% | 19.0 | 64%, 34%, 2% | 4.6 | |
High | 20%, 43%, 37% | 3%, 16%, 25%, 28%, 28% | 23%, 61%, 16% | 17.0 | 74%, 20%, 6% | 5.6 | |
Goodman = 0.44 (P < 0.001) | Goodman = 0.39 (P < 0.001) | Goodman = -0.06 (P = 0.238) | Cuzick trend (P = 0.010) | Goodman = -0.12 (P = 0.031) | Cuzick trend (P = 0.021) | ||
Cyclin D1 nuclear intensity | |||||||
Negative/low | 23%, 33%, 26%, 11%, 4% | 24%, 62%, 14% | 18.0 | 67%, 25%, 8% | 2.9 | ||
Intermediate | 1%, 12%, 41%, 36%, 10% | 18%, 65%, 17% | 18.0 | 68%, 25%, 7% | 4.8 | ||
High | 0%, 1%, 7%, 37%, 55% | 17%, 59%, 24% | 17.5 | 71%, 22%, 7% | 6.3 | ||
Goodman = 0.88 (P < 0.001) | Goodman = 0.16 (P < 0.001) | Cuzick trend (P = 0.553) | Goodman = -0.05 (P = 0.327) | Cuzick trend (P < 0.001) | |||
Cyclin D1 nuclear fraction | |||||||
< 1% | 18%, 68%, 14% | 20.0 | 2%, 29%, 9% | 2.8 | |||
1 to 9% | 23%, 62%, 15% | 18.0 | 68%, 25%, 7% | 2.7 | |||
10 to 32% | 24%, 59%, 17% | 18.0 | 71%, 25%, 4% | 4.4 | |||
33 to 67% | 20%, 59%, 21% | 18.0 | 70%, 21%, 9% | 4.9 | |||
> 67% | 12%, 68%, 20% | 16.0 | 68%, 24%, 8% | 5.9 | |||
Cuzick trend (P = 0.005) | Spearman ρ = -0.04 (P = 0.162) | Cuzick trend (P = 0.694) | Spearman ρ = 0.22 (P < 0.001 | ||||